FUW TRENDS IN SCIENCE & TECHNOLOGY JOURNAL

(A Peer Review Journal)
e–ISSN: 2408–5162; p–ISSN: 2048–5170

FUW TRENDS IN SCIENCE & TECHNOLOGY JOURNAL

ISOLATION AND CHARACTERIZATION OF STIGMASTEROL AND Β-SITOSTEROL FROM ETHYL ACETATE EXTRACT OF Adenodolichos paniculatus (FABACEAE)
Pages: 770-772
I. Ibrahim1, I. A. Bello, I. G. Ndukwe and I. G. Kizito


keywords: Introduction The increase in drug resistance recorded by path

Abstract

The leaves of Adenodolichos paniculatus (Fabaceae) was collected, identified, dried and pulverized. The pulverized plant material was subjected to microwave assisted extraction using n-hexane, chloroform, ethyl acetate and methanol. Column chromatography of the ethyl acetate extract led to a number of fractions. TLC fingerprinting and the spraying reagent (10% H2SO4) was used to identify the fraction containing phytosterols. The isolation and purification afforded a white crystalline powder which was subjected to physical (melting point determination), and spectroscopic identification using IR, 1H and 13C NMR. The compound was identified as a mixture of stigmasterol and β‐sitosterol.

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